Zona Laser Drill
Sperm Analyzer
Laser XY Clone
Live Cell Imaging
Special Slides
Anesthesia/
Veterinary Ventilator
IVF Application
 
Transgenic Applications

VelociMouse - NEW Publication! January 2007

F0 generation mice fully derived from gene-targeted embryonic stem cells allowing immediate phenotypic analyses.

William T Poueymirou, Wojtek Auerbach, David Frendewey, Joseph F Hickey, Jennifer M Escaravage, Lakeisha Esau, Anthony T Dore, Sean Stevens, Niels C Adams, Melissa G Dominguez, Nicholas W Gale, George D Yancopoulos, Thomas M DeChiara& David M Valenzuela.

Nature Biotechnology, 2007. Jan. 25(1):91-99.

A useful approach for exploring gene function involves generating mutant mice from genetically modified embryonic stem (ES) cells. Recent advances in genetic engineering of ES cells have shifted the bottleneck in this process to the generation of mice. Conventional injections of ES cells into blastocyst hosts produce F0 generation chimeras that are only partially derived from ES cells, requiring additional breeding to obtain mutant mice that can be phenotyped. The tetraploid complementation approach directly yields mice that are almost entirely derived from ES cells, but it is inefficient, works only with certain hybrid ES cell lines and suffers from nonspecific lethality and abnormalities, complicating phenotypic analyses. Here we show that laserassisted injection of either inbred or hybrid ES cells into eight cell-stage embryos efficiently yields F0 generation mice that are fully ES cell-derived and healthy, exhibit 100% germline transmission and allow immediate phenotypic analysis, greatly accelerating gene function assignment.

Oklahoma Medical Research Foundation

After receiving a demonstration of the XYClone at their laboratory, they learned that the laser could also replace the sharp micropipettes they used for blastocyst injection. When comparing methods, they find that the “insertion of cells into the blastocysts cavity is easier with the XYClone and causes less damage to the blastocysts” than with their old micropipette method.


Somatic Cell Nuclear Transfer (Testimonial)

Vanessa Hall
Senior Research Associate
University of Newcastle
Institute of Human Genetics
Department of Stem Cell Biology and Developmental Genetics


The XYClone laser has been of huge benefit to developing our technique of human somatic cell nuclear transfer. Prior to using the XYClone we were relying on traditional enucleation procedures to remove the genetic material from human oocytes. This generally requires piercing through the elastic outer protein shell of the egg by using a microscopic bevelled glass pipette, which often induced damage and resulted in lysis of the egg. The elasticity of the human zona pellucida compared with other species remained a challenge. However, following installation of the XYClone laser objective, we have been able to speed the process of enucleation to a matter of seconds, without eliciting damage to the egg. diseases.

The XYClone laser suitably makes a very small, neat slit in the zona pellucida which faciliates the DNA removal. We have been very impressed by how it has dramatically improved our enucleation efficiency and reduced the lysis rates of the eggs. It has also been incredibly easy to operate and we love how easy it is to target the site of interest using the software provided. It appears to be an extremely powerful tool that has improved our ability to create human cloned embryos for the purposes of deriving patient specific stem cell lines which may one day be used to treat non-curable.


Inner Cell Mass (ICM) Excision

Laser-assisted blastocyst dissection and subsequent cultivation of embryonic stem cells in a serum/cell free culture system: applications and preliminary results in a murine model.
Noriko Tanaka1, Takumi Takeuchi1, Queenie V Neri1, Eric Scott Sills2 and Gianpiero D Palermo1
1Center for Reproductive Medicine and Infertility, Weill Medical College of Cornell University, New York, NY 10021, USA
2Department of Obstetrics, Gynecology and Reproductive Research, Murphy Medical Center, Murphy, NC, USA
Journal of Translational Medicine 2006, 4 : 20    

Abstract from American Society of Reproductive Medicine, September 2005

A Feeder Cell- and Serum-Free System to Harvest Embryonic Stem Cells From Laser Dissected Blastocysts. T. Takeuchi, N. Tanaka, Q.V. Neri, Z. Rosenwaks, G.D. Palermo. Cornell University, New York, NY.

The XYClone was used for laser assisted ICM dissection, and preliminary data represents a feasible model to establish ESC lines in a controlled and xenogenic by-product free environment.

Maisam 'Maya' Mitalipova, Ph.D.

Research Scientist, Rhodes Center, University of Georgia. hES Consultant,

"Laser technology is very essential for isolation of human ES cells because it eliminates the use of animal product, such as antibodies for immunosurgery, and allows very precise separation of ICM from trophoblast, maintaining the intactness of inner cell mass. It is essential for xenofree ES cells.“

Video of ICM Isolation

IVF and ICSI

XYClone Provides a 42-46% Increase in Fertilization Rate for Mouse IVF with a B6 background

Courtesy of Kathy Mohr, Facility Director, Mutant Mouse Regional Resource Center, University of North Carolina at Chapel Hill

In an unpublished study, the fertilization rates of mouse (B6) oocytes were compared between two protocols: conventional mouse IVF, and oocyte zona breaching with the XYClone laser before exposure to spermatozoa (cryopreserved). The results showed that fertilization rates of oocytes in the XYClone group were 42-46% higher than those achieved using standard IVF methods.

XYClone™ Laser System: A Novel Technique for Mouse Intracytoplasmic Sperm Injection (ICSI)

William A. Richie, Embryologist, Division of Gene Expression and Development, Roslin Institute, Roslin Midlothian UK EH25 9PS and K. Bradley, Hamilton Thorne Biosciences, Beverly, MA 01915 USA

Introduction

A piezo actuated device has long been recognized as a necessary tool for the production of transgenic mice via intracytoplasmic sperm injection. Problems associated with
the time required to learn how to use a Piezo system efficiently, as well as the additional cost of purchasing pipettes and the handling and disposal of mercury are eliminated when using the XYClone laser system.

Goal

The purpose of this study was to investigate the efficiency of injection and development of mouse oocytes to blastocyst when the XYClone laser was utilized for membrane breaching instead of a piezo impact driver.

View Video of Mouse ICSI    

  View Video of Bovine ICSI



Laser Assisted Hatching

Increased Pregnancy Rate for Bovine Embryos

In a recent unpublished study, a 20% increase in pregnancy rate occurred when the XYClone was used for laser assisted hatching of bovine embryos.

Image below shows frozen / thawed bovine embryos before and after laser assisted hatching (courtesy of Michael E. Wehrman, Ph.D., Rocky Mountain Reproductive Services)

Before After